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hoechst 33258 dye solution  (Beyotime)
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Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
Hoechst 33258 Dye Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dye+hoechst/Hoechst+33258/pmc13122800-128-3-7
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hoechst 33258 dye solution - by Bioz Stars, 2026-09
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hoechst dye 33258  (Nacalai)
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Nacalai hoechst dye 33258
Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
Hoechst Dye 33258, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hoechst dye  (Double Helix)
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Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
Hoechst Dye, supplied by Double Helix, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell permeable dna dye hoechst 33342  (Beyotime)
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Beyotime cell permeable dna dye hoechst 33342
Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
Cell Permeable Dna Dye Hoechst 33342, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dye  (Beyotime)
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Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
Dye, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear dye hoechst 33342  (Beyotime)
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Beyotime nuclear dye hoechst 33342
Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
Nuclear Dye Hoechst 33342, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hoechst dye  (Fisher Scientific)
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Fisher Scientific hoechst dye
Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) <t>Hoechst</t> <t>33258</t> staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
Hoechst Dye, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) Hoechst 33258 staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: Cd-induced apoptosis of chicken primary kidney cells and the antagonistic effect of Tax. (A) AO/EB staining results of chicken kidney cells. Normal cells were green; apoptotic cells were orange; necrotic cells were red (scale bar, 200 μm). (B) Hoechst 33258 staining results of chicken kidney cells (scale bar, 200 μm). (C) Quantitative analysis of apoptotic cells detected by Hoechst 33258 staining. (D) The apoptosis level of primary kidney cells was detected by flow cytometry. (E) Quantitative analysis of the distribution of primary kidney cells in flow cytometry results. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group (n=10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Article Snippet: 300 μL of Hoechst 33258 dye solution (Beyotime Biotechnology, China) was added to each well to cover the cells, and the cells were stained in an incubator for 20 min (37 °C, 5 % CO 2 ).

Techniques: Staining, Flow Cytometry